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Electron Microscopy Facility
Subsidiary of the Research and Graduate Studies Office of the AVP

Transmission Electron Microscope Overview

Overview of the Transmission Electron Microscopes housed in the BYU Electron Microscopy Facility. Click on a microscope name for more details.

Transmission Electron Microscopes (TEM)

Tecnai TF20 TEM/STEMKrios G4 Cryo TEM/STEMIliad TEM/STEM

Bright Field

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X

X

Dark Field

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X

X

SAED

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X

X

CBED

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X

X

Micro ED

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STEM

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X

X

EDXA

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X

EELS

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X

EF TEM

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X

X

Tomography

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X

Cryo EM

X

SPA

X

Definitions

Bright Field – Imaging technique that uses the electrons that are transmitted through the sample for image formation. Scattered electrons provide contrast to an otherwise bright image.

Dark Field – Imaging technique that uses the selected scattered electrons from the sample for image formation.

SAED - Selected Area Electron Diffraction. Shows the diffraction pattern from a selected area of a sample.

CBED - Convergent Beam Electron Diffraction. Shows the diffraction pattern from a convergent electron beam.

Micro ED - Micro Electron Diffraction. Use of electron diffraction for determining crystallographic structure.

STEM - Scanning Transmission Electron Microscopy. This uses a highly demagnified electron beam to scan the sample to produce a serial image from the sample.

EDXA – Energy Dispersive X-ray Analysis is an analytical technique used for the elemental analysis or chemical characterization of a sample.

EELS - Electron Energy Loss Spectroscopy. Measures the energy loss from an electron after it has interacted with a sample to determine the sample's elemental composition and/or valence states between atoms.

Tomography - Process of taking multiple images through a tilt series to reconstruct a three dimensional image from a volume.

Cryo EM - Cryogenic Electron Microscopy. Keeping the sample at liquid nitrogen temperatures while observing it in the TEM. This preserves the sample structures for observation that would otherwise be lost with other observation techniques.

SPA - Single Particle Analysis. The automated process of taking a large number of images from a randomly dispersed protein sample that will be used to determine the protein conformation.